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Fig. 4 | Bioelectronic Medicine

Fig. 4

From: Low intensity trans-spinal focused ultrasound reduces mechanical sensitivity and suppresses spinal microglia activation in rats with chronic constriction injury

Fig. 4

Treatment with tsFUS in CCI animals reduces activated spinal microglia, ipsilateral to injury. A Flow cytometry gating strategy to detect activated spinal microglia. A1: Cells were first gated on singlets. A2: Only viable cells from singlets were analyzed. A3: Microglia were identified using the cell surface markers CD45 and CD11b/c + . A4: The activated of microglia were identified by CD86 expression (a biomarker of M1 phenotype). B Effect of CCI and tsFUS on number of activated spinal microglia. B1, Number of activated microglia from spinal segments L4 and L6, shown separately for the contralateral and ipsilateral sides to the CCI, in each testing group. Each dot represents individual rat. (t-test; p < 0.05). B2: Ratios of ipsilateral to contralateral counts of activated microglia. (ANOVA; p < 0.05)

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Bioelectronic Medicine

ISSN: 2332-8886