Fig. 3

Treatment with tsFUS in CCI animals reduces numbers of spinal microglia ipsilateral to injury. A Example IHC images showing microglia on spinal sections at L5 level labeled with Iba1. A1: Sham CCI animal, A2: CCI animal that received sham stimulation (CCI + sham FUS). A3: CCI animal that received tsFUS (CCI + tsFUS). The white box in each panel shows the region of interest (ROI) used to quantify microglia, separately for each side of the spinal cord; red line represents midline. A4-A6: Rotated and magnified images corresponding to ROIs from respective panels. Arrow indicates ipsilateral side to the CCI in each panel. B Quantification of the effect of CCI and tsFUS on number of spinal microglia. B1, number of microglia within the ROI, reported separately for contralateral and ipsilateral sides to the CCI, in sham CCI, CCI + FUS sham, and CCI + tsFUS animals. Each symbol represents an individual animal (paired t-test; p < 0.05 is deemed significant). B2: Ratios of the number of microglia on the ipsilateral to contralateral sides (ANOVA with group as independent variable, followed by pair-wise comparisons between groups; p < 0.05 corrected for multiple comparisons is deemed significant)