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Fig. 1 | Bioelectronic Medicine

Fig. 1

From: Low intensity trans-spinal focused ultrasound reduces mechanical sensitivity and suppresses spinal microglia activation in rats with chronic constriction injury

Fig. 1

Experimental setup and timeline. A Trans-spinal FUS (tsFUS) was transcutaneously targeted at the L5 segment level of the spinal cord. The color map shows the ultrasound field from the transducer within the spinal cord. B In all animals, treatment with tsFUS or sham stimulation was administered once daily for 3 min at days 4, 5 and 6 after chronic constriction injury (CCI). In a behavioral cohort (N = 6 tsFUS, N = 6 sham), von Frey threshold (VFT) testing was conducted 1 day before CCI (baseline), and at 4, 7, 9, 16 and 23 days after CCI. Some animals were sacrificed for immunohistochemistry (IHC: N = 5 for sham CCI, CCI + tsFUS sham, and CCI + tsFUS) or flow cytometry (FC: N = 5 for sham CCI, N = 4 for CCI + tsFUS sham and CCI + tsFUS), on day 6 after CCI. The cone of the sketch represents only the exit tip of the physical coupling cone

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Bioelectronic Medicine

ISSN: 2332-8886